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p jak1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p jak1
    P Jak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1+antibody/Phospho-Jak1+(Tyr1034%2F1035)+Antibody/pmc13039202-279-32-80
    Average 95 stars, based on 310 article reviews
    p jak1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cream:

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (Lot Number: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). .. Desktop High-speed Refrigerated Microcentrifuge (Model Number: D3024R, DLAB Scientific Co., Ltd), Microplate reader (Model No: RT-6100, Rayto), Vertical Electrophoresis System (Model Number: 1658001, BIO-RAD), Trans-Blot (Model Number: 1703930, BIO-RAD), Western Transfer (Model Number: 1704273, BIO-RAD), Gel Imaging system (Model Number: 12003254, BIO-RAD), and Upright White Light Photographic Microscope (Model Number: Eclipse Ci-L, Nikon).

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways.
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (LotNumber: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). ..

    Staining:

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (Lot Number: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). .. Desktop High-speed Refrigerated Microcentrifuge (Model Number: D3024R, DLAB Scientific Co., Ltd), Microplate reader (Model No: RT-6100, Rayto), Vertical Electrophoresis System (Model Number: 1658001, BIO-RAD), Trans-Blot (Model Number: 1703930, BIO-RAD), Western Transfer (Model Number: 1704273, BIO-RAD), Gel Imaging system (Model Number: 12003254, BIO-RAD), and Upright White Light Photographic Microscope (Model Number: Eclipse Ci-L, Nikon).

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways.
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (LotNumber: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). ..

    Protease Inhibitor:

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (Lot Number: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). .. Desktop High-speed Refrigerated Microcentrifuge (Model Number: D3024R, DLAB Scientific Co., Ltd), Microplate reader (Model No: RT-6100, Rayto), Vertical Electrophoresis System (Model Number: 1658001, BIO-RAD), Trans-Blot (Model Number: 1703930, BIO-RAD), Western Transfer (Model Number: 1704273, BIO-RAD), Gel Imaging system (Model Number: 12003254, BIO-RAD), and Upright White Light Photographic Microscope (Model Number: Eclipse Ci-L, Nikon).

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways.
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (LotNumber: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). ..

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Bicinchoninic Acid Protein Assay:

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (Lot Number: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). .. Desktop High-speed Refrigerated Microcentrifuge (Model Number: D3024R, DLAB Scientific Co., Ltd), Microplate reader (Model No: RT-6100, Rayto), Vertical Electrophoresis System (Model Number: 1658001, BIO-RAD), Trans-Blot (Model Number: 1703930, BIO-RAD), Western Transfer (Model Number: 1704273, BIO-RAD), Gel Imaging system (Model Number: 12003254, BIO-RAD), and Upright White Light Photographic Microscope (Model Number: Eclipse Ci-L, Nikon).

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways.
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (LotNumber: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (Lot Number: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). .. Desktop High-speed Refrigerated Microcentrifuge (Model Number: D3024R, DLAB Scientific Co., Ltd), Microplate reader (Model No: RT-6100, Rayto), Vertical Electrophoresis System (Model Number: 1658001, BIO-RAD), Trans-Blot (Model Number: 1703930, BIO-RAD), Western Transfer (Model Number: 1704273, BIO-RAD), Gel Imaging system (Model Number: 12003254, BIO-RAD), and Upright White Light Photographic Microscope (Model Number: Eclipse Ci-L, Nikon).

    Article Title: Efficacy of Medicated Thread Moxibustion of Zhuang Medicine on Skin Lesions in Eczema Rats Based on p38/NF-κB and JAK1-STAT6 Pathways.
    Article Snippet: .. 1-Chloro-2,4-dinitrobenzene (LotNumber: 237329, Sigma–Aldrich), acetone (Lot Number: 2021109, Chengdo Kelong Chemical Co., Ltd), olive oil (AGRIC), Pevisone cream (Lot Number: H20000454, Xian Janssen Pharmaceutical Ltd.), HE staining kit (Lot Number: C0105M, Shanghai Beyotime Biotechnology Co., Ltd), protein phosphatase inhibitors 3 (Lot Number: P0044, Sigma), protease inhibitor (Lot Number: P8640, Sigma), BCA protein assay kit (Lot Number: 23225, ThermoFisher), 4%–15% protein gradient gel (Lot Number: 5671085, BIO-RAD), anti-JAK1 antibody (Lot Number: AF5012, AFFINITY), p-JAK1 antibody (Lot Number: AF2012, AFFINITY), anti-STAT6 antibody (Lot Number: AF6302, AFFINITY), p-STAT6 antibody (Lot Number: AF3301, AFFINITY), β-tubulin antibody (Lot Number: 2146s, CST), p-P38 antibody (Lot Number: AF2006, AFFINITY), NF-κB p65 antibody (Lot Number: AF5006, AFFINITY), p-NF-κB p65 antibody (Lot Number: AF2006, AFFINITY), GAPDH antibody (Lot Number: AF7021, AFFINITY), rat IL-1β kit (Lot Number: ED-30206, Xiamen Lunchangshuo Biotechnology Co., Ltd), and rat IL-4 ELISA Kit (Lot Number: ED-30217, Xiamen Lunchangshuo Biotechnology Co., Ltd). ..

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Lysis:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    SDS Page:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Protein Concentration:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Recombinant:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Adjuvant:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Transfection:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Real-time Polymerase Chain Reaction:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.

    Polymerase Chain Reaction:

    Article Title: Shikonin attenuates rheumatoid arthritis by targeting SOCS1/JAK/STAT signaling pathway of fibroblast like synoviocytes
    Article Snippet: The powder of SKN (purity > 98%) and MTX were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Fetal bovine serum and H-DMEM were obtained from GIBCO; Tetrazolium bromide reduction (MTT) was purchased from Sigma. .. Protease inhibitor, mixed lysis buffer, protein phosphatase inhibitors, and hypersensitive luminescent solution were achived from Beijing Puli Lai Gene Technology Co. Ltd. SDS-PAGE protein buffer (5 ×) and Bradford protein concentration assay kit from Biyun Tian biotechnology; Matrigel was obtained from Corning; Transwell chambers and recombinant human TNF-α from Costar and PeproTech respectively; horseradish enzyme-labelled goat anti-rabbit antibody and adhesion protein from Millipore; anti-fluorescence quenching coating agent from Southern Biotech; Dimethyl sulfoxide (DMSO) was purchased from Amresco; SDS, crystal violet, glycine and Tris alkali powder were purchased from the meimen Lun Biotechnology Co. Ltd.; DAPI was purchased from AAT Bioquest company; complete and incomplete Freund’s adjuvant (C/IFA), bovine type II collagen were got from Chondrex; mouse IL-6, mouse TNF-α and mouse IL-1β ELISA kits from ABclonal; phosphorylated (p)-STAT6 antibody and p-JAK1 antibody were purchased from LifeSpan Biological Sciences and Cell Signaling Technology respectively; JAK1 antibody, anti-STAT6, anti-STAT1, p-STAT1 antibody, anti-GAPDH and anti-Vimentin antibodies were got from Abcam; TNF-α, IL-1β and IL-6 antibody were got from Abclonal; RiboFECTTM CP Transfection Kit were from RIOBIO; All real-time quantitative Polymerase Chain Reaction (PCR) reagents were purchased from OMEGA. .. Male DBA/1 mice (6–8 weeks) were purchased from Shanghai SLAC Experimental Animal Co. Ltd with production license No: SCXK 2017-0005.



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    Image Search Results


    EFHD2 influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of JAK and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.

    Journal: CytoJournal

    Article Title: Mapping the function of EF-hand domain-containing protein 2 and determining its clinical relevance in non-small-cell lung cancer through single-cell transcriptomics

    doi: 10.25259/Cytojournal_29_2025

    Figure Lengend Snippet: EFHD2 influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of JAK and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.

    Article Snippet: The membranes were then exposed overnight at 4°C to primary antibodies targeting EFHD2 (1:1000, ab24368, Abcam, China), JAK1 (1:1000, HY- P80196 , MedChemExpress, China), phospho-JAK (p-JAK) (1:1000, ab138005, Abcam, China), signal transducers and activators of transcription 3 (STAT3) (1:1000, ab68153, Abcam, China), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000, ab8245, Abcam, China) (loading control).

    Techniques: Migration, Protein-Protein interactions, Expressing, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Colony Assay, Control, Western Blot, Real-time Polymerase Chain Reaction, Cell Counting, Over Expression, Knockdown, Standard Deviation

    EFHD2 regulates cell proliferation, migration, invasion, apoptosis, and JAK/STAT signaling. (a) Co-IP identified that FLAG-EFHD2 interacted with Myc-JAK1 in A549+EFHD2 OE and NCI-H1299+EFHD2 KD cells. (b) CCK-8 detected cell proliferation. Inhibition of JAK decreased the proliferation of A549+EFHD2 OE cells, while activation of JAK increased the proliferation of NCI-H1299+EFHD2 KD cells. (c) Flow cytometry to detect apoptosis. Inhibition of JAK increased the apoptosis of A549+EFHD2 OE cells, whereas JAK agonist enhanced the apoptosis of NCI-H1299+EFHD2 KD cells. (d) Transwell migration and invasion. Inhibition of JAK reduced the migration and invasion of A549+EFHD2 OE cells, whereas activation of JAK promoted the migration and invasion of NCI-H1299+EFHD2 KD cells. (e) Colony formation assay. Inhibition of JAK decreased the colony formation of A549+EFHD2 OE cells, whereas activation of JAK enhanced the colony formation of NCI-H1299+EFHD2 KD cells. (f) Western blotting. JAK inhibition decreased the levels of p-JAK1 and p-STAT3 in A549+EFHD2 OE cells, whereas JAK activation increased the levels of p-JAK1 and p-STAT3 in NCI-H1299+EFHD2 KD cells. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, Co-IP: Co-immunoprecipitation, EFHD2: EF-hand domain-containing protein 2, OE: Overexpression, KD: Knockdown, CCK-8: Cell counting kit 8, p-JAK: Phospho Janus Kinase, p-STAT: Phospho signal transducers and activators of transcription, SD: Standard deviation.

    Journal: CytoJournal

    Article Title: Mapping the function of EF-hand domain-containing protein 2 and determining its clinical relevance in non-small-cell lung cancer through single-cell transcriptomics

    doi: 10.25259/Cytojournal_29_2025

    Figure Lengend Snippet: EFHD2 regulates cell proliferation, migration, invasion, apoptosis, and JAK/STAT signaling. (a) Co-IP identified that FLAG-EFHD2 interacted with Myc-JAK1 in A549+EFHD2 OE and NCI-H1299+EFHD2 KD cells. (b) CCK-8 detected cell proliferation. Inhibition of JAK decreased the proliferation of A549+EFHD2 OE cells, while activation of JAK increased the proliferation of NCI-H1299+EFHD2 KD cells. (c) Flow cytometry to detect apoptosis. Inhibition of JAK increased the apoptosis of A549+EFHD2 OE cells, whereas JAK agonist enhanced the apoptosis of NCI-H1299+EFHD2 KD cells. (d) Transwell migration and invasion. Inhibition of JAK reduced the migration and invasion of A549+EFHD2 OE cells, whereas activation of JAK promoted the migration and invasion of NCI-H1299+EFHD2 KD cells. (e) Colony formation assay. Inhibition of JAK decreased the colony formation of A549+EFHD2 OE cells, whereas activation of JAK enhanced the colony formation of NCI-H1299+EFHD2 KD cells. (f) Western blotting. JAK inhibition decreased the levels of p-JAK1 and p-STAT3 in A549+EFHD2 OE cells, whereas JAK activation increased the levels of p-JAK1 and p-STAT3 in NCI-H1299+EFHD2 KD cells. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, Co-IP: Co-immunoprecipitation, EFHD2: EF-hand domain-containing protein 2, OE: Overexpression, KD: Knockdown, CCK-8: Cell counting kit 8, p-JAK: Phospho Janus Kinase, p-STAT: Phospho signal transducers and activators of transcription, SD: Standard deviation.

    Article Snippet: The membranes were then exposed overnight at 4°C to primary antibodies targeting EFHD2 (1:1000, ab24368, Abcam, China), JAK1 (1:1000, HY- P80196 , MedChemExpress, China), phospho-JAK (p-JAK) (1:1000, ab138005, Abcam, China), signal transducers and activators of transcription 3 (STAT3) (1:1000, ab68153, Abcam, China), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000, ab8245, Abcam, China) (loading control).

    Techniques: Migration, Co-Immunoprecipitation Assay, CCK-8 Assay, Inhibition, Activation Assay, Flow Cytometry, Colony Assay, Western Blot, Immunoprecipitation, Over Expression, Knockdown, Cell Counting, Standard Deviation